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Protocols

cDNA synthesis

Cell culture

Cryosectioning

Designing primers

Designing qPCR primers

Gel extraction

Genomic DNA extraction

In situ hybridization

Melatonin assay

Muscle cellarity analysis

Muscle cell culture

Normalization of raw data with GeNorm & NormFinder

PCR

Quantitative real-time PCR (qPCR)

RNA extraction

Sampling tissues

Sequencing

Transformation

Vector ligation

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